Simplified HPLC method for identification of gingerol and mangiferin in herbal extracts

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European Journal of Scientific Research


Quantification of bioactive compounds through modern analytical techniques is essential for proving authenticity, credibility, and safe use of herbal products in Ayurvedic medicine. In this study, we describe the development of a simplified method for the identification of key bioactive compounds in six commercial herbal products of various forms using High Performance Liquid Chromatography (HPLC). Marker compounds such as mangiferin 1 and [6]-gingerol 2 were examined in various extracts of mango leaves and ginger, respectively. The compounds were extracted by sonication with methanol at room temperature and were analyzed by HPLC with 0.1% acetic acid buffer (pH = 3.00)/methanol mixture used as gradient mobile phase. Eluents were monitored by a photo diode array (PDA) detector and UV detection fixed at 280/320 nm. Further characterization of each extract was performed using FTIR and UV-Vis. Mangiferin 1 eluted at 6 min (320 nm) and gingerol 2 at 16.9 min (280 nm). The proposed method provides a good resolution of the selected marker compounds in herbal products and dietary supplements that could be further used for their quantification and standardization in routine analysis and quality control. © EuroJournals Publishing, Inc. 2011.

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