Monitoring bacterial diversity in a full-scale municipal wastewater treatment plant in dubai by fluorescence in situ hybridization technique
Document Type
Article
Source of Publication
International Journal of Environmental Research
Publication Date
4-11-2013
Abstract
In this study, the bacterial diversity in the activated sludge system of a full-scale municipal wastewater treatment plant in Dubai was monitored over a period of one year using ribosomal RNA (rRNA) targeted oligonucleotide probes for a defined phylogenetic group of bacteria by the Fluorescence in situ hybridization (FISH) technique. The largest fraction of the bacterial community in the sludge samples belonged to the gamma-subgroup of proteobacteria (25%) followed by gram positive bacteria of high G+C content(16%), gram positive bacteria with low G+C content (9 %), beta-proteobacteria (8%) and alpha-proteobacteria (5 %) with respect to the population percentages stained by DAPI (4,6-diamino-2-phenylindole). A specific nocardioform actinomycete, simultaneously targeted by both HGC69a and MNP1 probes, was predominantly found throughout the study period in all activated sludge mixed liquor samples. The nocardioform actinomycetes group members were detected in both branched and single cell morphotypes. Most of the previously published genus and species specific probes failed to hybridize to the sludge samples. In conclusion, the overall bacterial community populations detected by the sub-group specific 16S rRNA targeted oligonucleotide probes in FISH technique remained almost constant throughout the period of study irrespective of treatment plant conditions.
ISSN
Volume
7
Issue
2
First Page
479
Last Page
484
Disciplines
Civil and Environmental Engineering
Keywords
Activated sludge, Bacterial community, Fluorescence in situ hybridization, Nocardioform actinomycetes, Oligonucleotide probes
Scopus ID
Recommended Citation
Khan, M. A. and Faheem, S. M., "Monitoring bacterial diversity in a full-scale municipal wastewater treatment plant in dubai by fluorescence in situ hybridization technique" (2013). All Works. 2438.
https://zuscholars.zu.ac.ae/works/2438
Indexed in Scopus
yes
Open Access
no