Next-generation sequencing (NGS) platforms: An exciting era of genome sequence analysis

Document Type

Book Chapter

Source of Publication

Microbial Genomics in Sustainable Agroecosystems: Volume 2

Publication Date

1-1-2019

Abstract

© Springer Nature Singapore Pte Ltd. 2019. DNA referred to as blueprint of life codes for the diversity and function of all the living organisms. Determining DNA sequences of the living organisms not only gives an overview of their genetic makeup, but also provides information about their function. Nonetheless it was not easy to determine the genome sequencing of all the diversity around us especially with the technologies available before 2010. Therefore, determining the sequence of humans and some other organisms only was prioritized. Pioneering methods for DNA sequencing given discovered by Maxam and Gilbert, and Sanger although were very powerful and popular but were not high throughput and economic. Therefore, it was necessary to develop new economic and high-throughput methods that can sequence the biodiversity consequently providing better insights of their possible function. New methods were developed and commercialized by Roche Life Sciences, Thermo Fisher Scientific, Illumina, and Applied Biosystems. These methods generally referred to as next-generation sequencing methods have revolutionized the DNA sequencing. Many sequencing platforms employing NGS have been developed including pyrosequencing, Ion Torrent technology, Illumina/Solexa platform, and SOLiD (Sequencing by Oligonucleotide Ligation and Detection). Further optimization has led to innovative third and fourth-generation platforms as single molecule real-time (SMRT) sequencing by PacBio, nanopore sequencing, etc. As a consequence there is a sharp increase in the number of genomes being published and other genome-based studies since 2012. This has made it easy even to imagine of sequencing the genomes of individuals. Furthermore, scientists are now looking for third-generation sequencers that may be significantly different from the sequencers that are currently available.

ISBN

9789813298606

Publisher

Springer Singapore

First Page

89

Last Page

109

Disciplines

Life Sciences

Keywords

Genome, Next-generation sequencing (NGS), NGS applications, NGS chemistry, NGS platforms

Scopus ID

85085419057

Indexed in Scopus

yes

Open Access

no

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