ORCID Identifiers

0000-0001-8369-5271

Document Type

Article

Source of Publication

Nutrients

Publication Date

6-1-2020

Abstract

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The composition of human breast milk is affected by several factors, including genetics, geographic location and maternal nutrition. This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota. A total of 30 breast milk samples were collected from healthy Emirati and UAE-expatriates at Latifa Hospital. HMO profiling was performed using UHPLC-MS. Microbiota profiles were determined by sequencing amplicons of the V3-V4 region of the 16S rRNA gene. HMO concentrations were significantly higher in Emirati, and dropped with the lactation period in both groups of mothers. The Le (a− b+)-secretor (Le+ Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a− b−)-secretor (Le− Se+) type (23%). Bifidobacterium and Lactobacillus were considerably lower in Dubai-based mothers, while Pseudomonas and Delftia (Hydrogenophaga) were detected at a higher abundance compared to mothers from other countries. Atopobium was correlated with sialyl-lacto-N-tetraose c, Leptotrichia and Veillonella were correlated with 6’-sialyl-lactose, and Porphyromonas was correlated with lacto-N-hexaose. The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition. Targeted genomic analyses may help in determining whether these differences are due to genetic variations or to sociocultural and environmental factors.

ISSN

2072-6643

Publisher

MDPI AG

Volume

12

Issue

6

First Page

1

Last Page

15

Disciplines

Life Sciences

Keywords

Breastfeeding mothers, Human milk oligosaccharides, Microbiota, Next generation sequencing, V3-V4 16S rRNA gene

Scopus ID

85086092148

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Indexed in Scopus

yes

Open Access

yes

Open Access Type

Gold: This publication is openly available in an open access journal/series

Included in

Life Sciences Commons

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