Document Type
Article
Source of Publication
International Journal of Infectious Diseases
Publication Date
12-1-2014
Abstract
© 2014 The Authors. Objectives: To detect, characterize, and assess the genetic clonality of carbapenem-non-susceptible Enterobacteriaceae in 10 Lebanese hospitals in 2012. Methods: Selected Enterobacteriaceae isolates with reduced susceptibility to carbapenems were subject to phenotypic study including antibiotic susceptibility, cloxacillin effect, modified Hodge test, and activity of efflux pump inhibitor. Carbapenemase genes were detected using PCR; clonal relatedness was studied by pulsed field gel electrophoresis. Results: Out of 8717 Enterobacteriaceae isolated in 2012, 102 (1.2%) showed reduced susceptibility to carbapenems. Thirty-one (70%) of the 44 studied clinical isolates harbored blaOXA-48, including 15 Klebsiella pneumoniae, eight Escherichia coli, four Serratia marcescens, three Enterobacter cloacae, and one Morganella morganii. The majority of OXA-48 producers co-secreted an extended-spectrum beta-lactamase, while one had an acquired AmpC of the ACC type. In the non-OXA-48 producers, carbapenem resistance was attributed to the production of acquired AmpC cephalosporinases of MOX or CIT type, outer membrane impermeability, and/or efflux pump overproduction. DNA fingerprints revealed that OXA-48 producers were different, except for clonal relatedness among four K. pneumoniae, two E. coli, two E. cloacae, and three S. marcescens. Conclusions: Nosocomial carbapenem-non-susceptible Enterobacteriaceae are moderately spread in Lebanon and the predominant mechanism is OXA-48 production.
DOI Link
ISSN
Publisher
Elsevier
Volume
29
First Page
e139
Last Page
e144
Disciplines
Life Sciences
Keywords
AmpC, Carbapenem resistance, Enterobacteriaceae, OXA-48
Scopus ID
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Hammoudi, D.; Ayoub Moubareck, C.; Aires, J.; Adaime, A.; Barakat, A.; Fayad, N.; Hakime, N.; Houmani, M.; Itani, T.; Najjar, Z.; Suleiman, M.; Sarraf, R.; and Sarkis, D. Karam, "Countrywide spread of OXA-48 carbapenemase in Lebanon: Surveillance and genetic characterization of carbapenem-non-susceptible Enterobacteriaceae in 10 hospitals over a one-year period" (2014). All Works. 1110.
https://zuscholars.zu.ac.ae/works/1110
Indexed in Scopus
yes
Open Access
yes
Open Access Type
Gold: This publication is openly available in an open access journal/series