Document Type
Article
Source of Publication
Cell Calcium
Publication Date
4-1-2015
Abstract
© 2015 Elsevier Ltd. Cannabidiol (CBD), a major nonpsychotropic cannabinoid found in Cannabis plant, has been shown to influence cardiovascular functions under various physiological and pathological conditions. In the present study, the effects of CBD on contractility and electrophysiological properties of rat ventricular myocytes were investigated. Video edge detection was used to measure myocyte shortening. Intracellular Ca2+ was measured in cells loaded with the Ca2+ sensitive fluorescent indicator fura-2 AM. Whole-cell patch clamp was used to measure action potential and Ca2+ currents. Radioligand binding was employed to study pharmacological characteristics of CBD binding. CBD (1μM) caused a significant decrease in the amplitudes of electrically evoked myocyte shortening and Ca2+ transients. However, the amplitudes of caffeine-evoked Ca2+ transients and the rate of recovery of electrically evoked Ca2+ transients following caffeine application were not altered. CBD (1μM) significantly decreased the duration of APs. Further studies on L-type Ca2+ channels indicated that CBD inhibits these channels with IC50 of 0.1μM in a voltage-independent manner. Radioligand studies indicated that the specific binding of [3H]Isradipine, was not altered significantly by CBD. The results suggest that CBD depresses myocyte contractility by suppressing L-type Ca2+ channels at a site different than dihydropyridine binding site and inhibits excitation-contraction coupling in cardiomyocytes.
DOI Link
ISSN
Publisher
Churchill Livingstone
Volume
57
Issue
4
First Page
290
Last Page
299
Disciplines
Chemistry | Medicine and Health Sciences | Physics
Keywords
Calcium channels, Cannabidiol, Cannabinoid, Contraction, Intracellular calcium, Ventricular myocytes
Scopus ID
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Ali, Ramez M.; Al Kury, Lina T.; Yang, Keun Hang Susan; Qureshi, Anwar; Rajesh, Mohanraj; Galadari, Sehamuddin; Shuba, Yaroslav M.; Howarth, Frank Christopher; and Oz, Murat, "Effects of cannabidiol on contractions and calcium signaling in rat ventricular myocytes" (2015). All Works. 1415.
https://zuscholars.zu.ac.ae/works/1415
Indexed in Scopus
yes
Open Access
yes
Open Access Type
Green: A manuscript of this publication is openly available in a repository