From Waste to Sensor: Facile Synthesis of a Copper-Doped Hydroxyapatite Nanocomposite as a Colorimetric Sensing Platform for Uric Acid

Document Type

Article

Source of Publication

ChemistrySelect

Publication Date

8-20-2024

Abstract

Uric acid serves as a vital diagnostic marker for diseases like arthritis, gout, etc. Accurate quantification of UA is crucial for diagnosis. The hydroxyapatite (HAp), being catalytic in nature, was used as a template for copper nanoparticles (CuNPs) synthesis and for providing surface area for chemical reactions needed for sensing uric acid. HAp was synthesized from waste chicken bones, followed by doping with copper metal salt through calcination. The synthesis of copper hydroxyapatite (Cu-HAp) nanocomposite was confirmed using different techniques. SEM displayed the nanoporous morphology of the material. The hexagonal crystal structure of HAp was revealed by X-ray diffraction analysis, and energy dispersive X-ray analysis showed the successful doping of Cu on HAp. The Cu-HAp nanocomposite and 3,3′,5,5′-tetramethylbenzidine (TMB) dye were employed as a new colorimetric sensing platform for uric acid sensing. The fabricated sensing platform detected uric acid with excellent sensitivity (LOD, 0.24 μM) and a wide linear range (1–270 μM). Optimization experiments demonstrated that the proposed sensor performs best at 4 mg of Cu-HAp nanocomposite, pH 6, with a response time of 100 s. Furthermore, TMB and H2O2 concentrations of 12 and 8 mM were used, respectively. The proposed sensor successfully detected uric acid in physiological samples.

ISSN

2365-6549

Publisher

Wiley

Volume

9

Issue

31

Disciplines

Environmental Chemistry

Keywords

Colorimetric sensing, Cu-HAp nanocomposite, Gout, TMB, Uric acid

Scopus ID

85201198144

Indexed in Scopus

yes

Open Access

no

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