Therapeutic Applications of Microbial Enzymes Recent Innovations and Future Prospects

Lakshmi Kesari Ambika, Zayed University

Document Type

Article

Source of Publication

Microbial Enzymes as Potential Biotherapeutics in Human Healthcare

Publication Date

1-1-2025

Abstract

Microbial enzymes are proteins synthesized by microorganisms that catalyze specific biochemical reactions and have garnered significant attention as potential therapeutic agents, owing to their diverse applications and unique properties. Various techniques have been developed for manipulating microbial enzymes, including protein engineering, directed evolution, rational design, immobilization, enzyme fusion, and encapsulation. However, several limitations persist, including immunogenicity, stability, specificity, production costs, delivery challenges, and regulatory hurdles. The field of enzyme engineering and production is experiencing a surge in innovative approaches to overcome the existing limitations. This chapter endeavors to explore the diverse array of cutting-edge methodologies currently employed to enhance enzyme performance and production efficiency. This research elucidates the potential of microbial enzymes as therapeutic agents and delineates various techniques for their manipulation, while acknowledging persistent challenges. The ongoing advancements in enzyme engineering and production methods hold promise for overcoming current limitations, potentially revolutionizing the field of enzyme-based therapeutics. These include the application of machine learning algorithms for enzyme design optimization, in silico screening techniques for rapid candidate identification, and CRISPR-Cas9 technology for precise genetic modifications. Researchers are also exploring innovative formulation strategies, including the use of nanoparticles, to improve enzyme delivery and efficacy in various applications. These advancements in enzyme engineering and production techniques have the potential to revolutionize industries ranging from pharmaceuticals to biofuels. By enhancing enzyme stability, activity, and delivery, these innovations could lead to more efficient and cost-effective processes in biotechnology and healthcare. Furthermore, the development of novel enzymatic pathways and improved production methods may pave the way for new therapeutic approaches and sustainable manufacturing solutions.

ISBN

[9781032752259, 9781040382615]

Publisher

CRC Press

First Page

12

Last Page

27

Disciplines

Medicine and Health Sciences

Keywords

Microbial enzymes, Therapeutic agents, Enzyme engineering, Biochemical reactions, Delivery challenges

Scopus ID

105013696960

Indexed in Scopus

yes

Open Access

no

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