An overview of real-time quantitative PCR: Principles and formats for environmental microbiology studies

Document Type

Article

Source of Publication

Asian Journal of Microbiology, Biotechnology and Environmental Sciences

Publication Date

2-1-2018

Abstract

© Global Science Publications. A fluorescence-based real-time quantitative polymerase chain reaction (qPCR) is a powerful and commonly used molecular technique for quantifying the rRNA or DNA of targeted organisms in environmental samples. qPCR assays are easy to perform, making them capable of high throughput and can combine high sensitivity with reliable specificity. qPCR analysis is the combination of the traditional endpoint PCR attached with fluorescents to record the accumulation of the amplicons in real time during each cycle of the PCR. Detection of amplicons during the early exponential phase enables quantification of the gene numbers because they are proportional to the starting template. This review is focussed on currently used qPCR platforms, the chemistries involved in real-time PCR systems mainly applied for the environmental microbiology studies. The various factors affecting quantification of environmental microbial communities using qPCR have also been discussed.

ISSN

0972-3005

Publisher

Global Science Publications

Volume

20

First Page

S34

Last Page

S43

Disciplines

Environmental Sciences | Life Sciences

Keywords

Environmental DNA, QPCR chemistries, QPCR formats, Quantitative polymerase chain reaction (qPCR)

Scopus ID

85045097185

Indexed in Scopus

yes

Open Access

no

This document is currently not available here.

Share

COinS