Applications of real-time quantitative PCR for environmental microbiology studies

Document Type

Article

Source of Publication

Asian Journal of Microbiology, Biotechnology and Environmental Sciences

Publication Date

2-1-2018

Abstract

© Global Science Publications. The real-time quantitative polymerase chain reaction (qPCR) is a robust and sensitive molecular technique to quantify phylogenetic and key functional genes of microbial populations in various natural and engineered environmental ecosystems under varying physical and chemical environmental conditions. The generated quantitative qPCR data can be used to relate variation in abundance of specific microbial groups, species, family or functional gene of interest and levels of gene expression in comparison with the change in environmental factors and or system processes. In this review, several applications of qPCR for the understanding of environmental microorganisms have been presented. The main qPCR applications reviewed are: quantification of uncultured environmental bacteria, detection of pathogens in environmental samples, the effect of antibiotics on anaerobic digester microbial community and understanding mechanism of nitrification and ammonia metabolism in engineered environmental ecosystems.

ISSN

0972-3005

Publisher

Global Science Publications

Volume

20

First Page

S44

Last Page

S51

Disciplines

Environmental Sciences | Life Sciences

Keywords

Environmental applications, Microbial communities, Molecular technique, Quantitative polymerase chain reaction (qPCR)

Scopus ID

85045099271

Indexed in Scopus

yes

Open Access

no

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