Applications of real-time quantitative PCR for environmental microbiology studies
Source of Publication
Asian Journal of Microbiology, Biotechnology and Environmental Sciences
© Global Science Publications. The real-time quantitative polymerase chain reaction (qPCR) is a robust and sensitive molecular technique to quantify phylogenetic and key functional genes of microbial populations in various natural and engineered environmental ecosystems under varying physical and chemical environmental conditions. The generated quantitative qPCR data can be used to relate variation in abundance of specific microbial groups, species, family or functional gene of interest and levels of gene expression in comparison with the change in environmental factors and or system processes. In this review, several applications of qPCR for the understanding of environmental microorganisms have been presented. The main qPCR applications reviewed are: quantification of uncultured environmental bacteria, detection of pathogens in environmental samples, the effect of antibiotics on anaerobic digester microbial community and understanding mechanism of nitrification and ammonia metabolism in engineered environmental ecosystems.
Global Science Publications
Environmental Sciences | Life Sciences
Environmental applications, Microbial communities, Molecular technique, Quantitative polymerase chain reaction (qPCR)
Khan, M. A.; Ganesh, A. G.; and Rais, Naushad, "Applications of real-time quantitative PCR for environmental microbiology studies" (2018). All Works. 522.
Indexed in Scopus